Concentration dependent chromatin states induced by the bicoid morphogen gradient

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Concentration dependent chromatin states induced by the bicoid morphogen gradient

In Drosophila, graded expression of the maternal transcription factor Bicoid (Bcd) provides positional information to activate target genes at different positions along the anterior-posterior axis. We have measured the genome-wide binding profile of Bcd using ChIP-seq in embryos expressing single, uniform levels of Bcd protein, and grouped Bcd-bound targets into four classes based on occupancy ...

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The gradient morphogen bicoid is a concentration-dependent transcriptional activator.

The bicoid (bcd) protein is expressed in an anteroposterior gradient in early Drosophila embryos and controls the zygotic activation of the segmentation gene hunchback (hb) in a broad but precisely bounded anterior domain. Here we show that the hb gene contains multiple regulatory elements that mediate transcriptional activation in response to bcd protein. Further, we demonstrate that the resul...

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Bicoid by the Numbers: Quantifying a Morphogen Gradient

Morphogen gradients are typically analyzed from static images of fixed embryonic tissues. Two papers in this issue of Cell now report live imaging of the Bicoid gradient in developing fruit fly embryos (Gregor et al., 2007a, 2007b). Their findings indicate that the gradient is highly reproducible from embryo to embryo and reveal that the nuclear dynamics of Bicoid are critical for maintaining p...

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Determining the scale of the Bicoid morphogen gradient.

Bicoid is a morphogen that sets up the anterior-posterior axis in early Drosophila embryos. Although the form of the Bicoid profile is consistent with a simple diffusion/degradation model, the observed length scale is much larger than should be expected based on the measured diffusion rate. Here, we study two possible mechanisms that could, in principle, affect this gradient and, hence, address...

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ژورنال

عنوان ژورنال: eLife

سال: 2017

ISSN: 2050-084X

DOI: 10.7554/elife.28275